Frequently Asked Questions

 Question or problem

Answer

Does the constraint of the micropattern geometry change cell physiology in any way?

Cells can suffer if the micropattern size is too small. Once the optimal pattern size is found (see question 3), cells go through their cell division cycle at the usual pace. Check our publication list for additional information on assays already carried out on our micropatterns.

Cells who need cell-cell contacts may also suffer on our current micropatterns. Contact us. We can offer custom made micropatterns for your special needs.

What kind of assay can be carried out on micropatterns?

Replace your coverslip or your glass bottom microplate with a CYTOOchip^TM or a CYTOOplate^TM and carry out your usual cell assay with minor modifications (we'll send you a detailed protocol for optimal cell seeding and spreading). Fix or video record during the first cell division cycle. Use your usual drugs, labels and probes..

How do I choose the best micropattern for my cell assay ?

Use our Starter's CYTOOchip^TM which offers 4 standard micropattern geometries in 3 sizes. Perform your cell assay on the Starter's CYTOOchip^TM and analyze which micropattern works best.
Description of micropatterns and uses (link) may also bring useful information.

How do I know if a micropattern is too small ?

Cells do not spread out on the micropatterns. They keep a round shape and are bright in phase contrast.
Cells may also make blebs.

How do I know if a micropattern is too big ?

Cells adopt various shapes on a given geometry.
In time lapse microscopy, cells never seem to stabilize on the micropattern but rather move back and forth. This induces a large variability in cell phenotypes.

My cells don't attach.

Wait longer. Some cell types take more than two hours to start spreading on micropatterns. In this case lower cell density to avoid cell aggregation.