CYTOOplates are high resolution glass-bottom microplates with thousands of adhesive micropatterns arrayed in each well.
When cells are seeded on concave micropatterns, the distribution of controlled adhesive contacts between cells and substrate induces the reproducible polarization of the cell machinery.
Cell shape, cell polarity and internal cell organization become normalized.
Standard shapes include Disc, Crossbow, H, Y and L in three sizes.
A CYTOOplate Starter's featuring different geometries is ideal to identify which micropattern works best for your assay and cell line.
Micropatterns are available in fibronectin coated (fluorescently labeled or not) or as ready-to-coat surfaces.
Other protein coatings are available as a custom service. Contact us for your special needs.
Product sheet• Powerful assay standardization
• Reproducible & sensitive cell phenotype quantification
• Straightforward image analysis
• Breakthrough Quantification: the Reference Cell™• Cell arraying and isolation
• Cytoskeleton rearrangement assay
• Cell polarity assay
• Organelle positioning assay
• Receptor internalization assay
• Cell division and spindle orientation assays
• Cell interaction assays
• Stem cell differentiation
• Ciliogenesis assay
Single Cell Normalization
Once cell seeding density is optimized, approximately 60 to 70% of cell population are normalized cells occupying up to 30% of micropatterns (SCORe= Single Cell Occupancy Rate). Some micropatterns are empty, some occupied by 2 or 3 cells. Normalized (single cells) can be easily identified and selected for further analysis by counting nuclei.
Download our Application Note to learn more.
The Reference Cell™: powerful phenotyping tool for HCS
The Reference Cell is a unique HCA methodology allowing comparability between reproducible and standardized assays.
From as few as 50 normalized cells, it is now possible to convert a stack of images into a single representative image of the cell population phenotype: the Reference Cell. Learn more here.
Generation of the Reference Cell for F-actin by averaging over a stack of aligned individual cells
Examples of F actin Reference Cells after drug treatment
(from left to right: Nocodazole 5µM, Blebbistatin 5µM, Y27632 5µM and Cytochalasin D 5µM)
Download Full Application Note here
Adhesive protein coatings
All CYTOOplates are available as Ready-to-use or Ready-to-coat.
Ready-to-use micropatterns:
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Ready-to-coat micropatterns:
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